Journal: Viruses
Article Title: Commercially Available Flavonols Are Better SARS-CoV-2 Inhibitors than Isoflavone and Flavones
doi: 10.3390/v14071458
Figure Lengend Snippet: ( A ) Inhibition percentage of flavonoids (10 μM) to 88.8 nM M pro . Blue, green, and red bars correspond to isoflavone, flavones, and flavonols, respectively. ( B ) The enzymatic inhibition profile for fisetin, myricetin, and GC376 (positive control) for 88.8 nM M pro in PBS. ( C ) Michaelis–Menten enzymatic mechanism for M pro in the absence and presence of a fixed fisetin or myricetin concentration (2.5 µM) at different substrate concentrations.
Article Snippet: Genistein, apigenin, luteolin, fisetin, kaempferol, myricetin, quercetin, remdesivir (RDV), carboxymethyl cellulose (CMC), tris(4-(dimethylamino)phenyl)methylium chloride (crystal violet), formaldehyde phosphate buffer solution (PBS), ethanol, dimethyl sulfoxide (DMSO), sulfuric acid (H 2 SO 4 ), and 3,3’,5,5’-tetramethylbenzidine (TMB) were purchased from Sigma-Aldrich/Merck (St. Louis, MO, USA).
Techniques: Inhibition, Positive Control, Concentration Assay